Molecular Weight Distribution of [beta]-Glucan in Oat-Based Foods

Cereal Chemistry | May 1, 2004| | Copyright

ABSTRACT

Oats, different oat fractions as well as experimental and commercial oat-based foods, were extracted with hot water containing thermostable [alpha]-amylase. Average molecular weight and molecular weight distributions of [beta]-glucan in extracts were analyzed with a calibrated high-performance size-exclusion chromatography system with Calcofluor detection, specific for the [beta]-glucan. Oats, rolled oats, oat bran, and oat bran concentrates all had high Calcofluor average molecular weights (206 × 10^sup 4^ to 230 × 10^sup 4^ g/mol) and essentially monomodal distributions. Of the ...

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