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Quantitation of Pulmonary Transgene Expression with PET Imaging
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PET imaging represents a promising approach for noninvasive monitoring of reporter gene expression in living subjects. We evaluated the relationship between various methods of quantifying the imaging signal and in vitro assays of the expression of a PET reporter gene (a mutant Herpes simplex virus-1 thymidine kinase (mHSV1-tk); 9-(4-^sup 18^F-fluoro-3-hydroxymethylbutyl)guanine (^sup 18^F-FHBG) was used as the PET reporter probe. Methods: In 14 rats, pulmonary gene transfer was performed by i...
Related newspaper, magazine, and journal articles from HighBeam Research
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Noninvasive Monitoring of Target Gene Expression by Imaging Reporter Gene Expression in Living Animals Using Improved Bicistronic Vectors
The Journal of Nuclear Medicine
; Indirect, noninvasive imaging of therapeutic gene expression based on levels of reporter gene expression is a powerful tool to devise improved therapeutic strategies in cancer gene therapy. The use of bicistronic vectors carrying internal ribosome entry sites (IRESs) allows the coexpression of
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Molecular-Genetic Imaging Based on Reporter Gene Expression
The Journal of Nuclear Medicine
; Molecular imaging includes proteomic, metabolic, cellular biologic process, and genetic imaging. In a narrow sense, molecular imaging means genetic imaging and can be called molecular-genetic imaging. Imaging reporter genes play a leading role in molecular-genetic imaging. There are 3 major methods
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Imaging of adenoviral-directed herpes simplex virus type 1 thymidine kinase reporter gene expression in mice with radiolabeled ganciclovir
The Journal of Nuclear Medicine
; We are developing procedures to repeatedly and noninvasively image the expression of transplanted reporter genes in living animals and in patients, using PET. We have investigated the use of the Herpes Simplex Virus type 1 thymidine kinase gene (HSV1-tk) as a reporter gene and [8-^sup
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Repression of Gene Expression by HD2-type Histone Deacetylases and Function Analysis of HD2 Proteins in Arabidopsis
In Vitro Cellular & Developmental Biology
; P-1012 HD2-typc proteins belong to one class of histone deacetylascs that is plant specific and shows no sequence homology to other classes of histone deacetylases. We demonstrated that Arabidopsis HD2-type proteins, AIHD2A, AtHD2B and AIHD2C, can repress gene expression when targeted to the
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8-[(18)F]fluoropenciclovir: An improved reporter probe for imaging HSV1-tk reporter gene expression in vivo using PET
The Journal of Nuclear Medicine
; We have synthesized and evaluated 8-[8F]fluoropenciclovir (FPCV) and compared it with 8-[18F]fluoroganciclovir (FGCV) for monitoring the expression of herpes simplex virus type 1 thymidine kinase (HSVI -tk) reporter gene in cell culture and in vivo. Methods: C6 rat glioma cells stably transfected
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The IMEter Predicts an Intron's Ability to Boost Gene Expression
Plant Cell
; Most eukaryotic genes are interrupted by one or more introns that are transcribed and then removed by splicing before the mRNA exits the nucleus. Some intron-containing genes have much higher expression levels than intronless versions of the same gene (reviewed in Le Hir et al., 2003). The first
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Use of Bacterial Quorum-Sensing Components to Regulate Gene Expression in Plants1[W]
Plant Physiology
; We describe an efficient inducible system to regulate gene expression in plants based on quorum-sensing components found in Gram-negative bacteria such as Agrobacterium tumefaciens. These bacteria monitor their own population density by utilizing members of the N-acyl homoserine lactone family as
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Imaging pulmonary gene expression with positron emission tomography
American Journal of Respiratory and Critical Care Medicine
; We evaluated positron emission tomographic imaging of pulmonary transgene expression, using an enhanced mutant herpes simplex virus-1 thymidine kinase as the reporter gene, in the lungs of normal rats. Sixteen rats were studied 3 days after an intratracheal administration of 5 109 to 1 10^sup 11^
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Human pharmacokinetic and dosimetry studies of [(18)F]FHBG: A reporter probe for imaging herpes simplex virus type-1 thymidine kinase reporter gene expression
The Journal of Nuclear Medicine
; 9-[4sup 18^F]fluoro-3-hydroxymethyl)butyl]guanine sup 18^F]FHBG) has been used as a reporter probe to image expression of herpes simplex virus type-1 thymidine kinase (HSV1-tk) reporter gene in living animals. Our aim was to study the kinetics, biodistribution, stability, dosimetry, and safety of
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A Human-Derived Reporter Gene for Noninvasive Imaging in Humans: Mitochondrial Thymidine Kinase Type 2
The Journal of Nuclear Medicine
; A human-derived intrinsically nonimmunogenic reporter gene was tested for PET imaging of different molecular-genetic processes for potential clinical use. Methods: The human mitochondrial thymidine kinase type 2 (hTK2) reporter gene truncated at the N terminus 916;hTK2), alone or fused with green
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