Bioluminescence color determinants of Phrixothrix railroad-worm luciferases: Chimeric luciferases, site-directed mutagenesis of Arg 215 and guanidine effect

From: Photochemistry and Photobiology | Date: August 1, 2000| Author: | Copyright information

Bioluminescence Color Determinants of Phrixothrix Railroad-worm Luciferases: Chimeric Luciferases, Site-directed Mutagenesis of Arg 215 and Guanidine effect

ABSTRACT

Chimeric proteins were produced using the green lightemitting luciferase of Phrixothrix vivianii (PxGr: lambda^sub max^ = 548 nm) and the red light-emitting luciferase of Phrixothrix hirtus (PxRe: lambda^sub max^= 623 nm). Constructs containing residues 1-344 of the red light-emitting luciferase with residues 345-545 of the green light emitting one emitted red light (PxReGr; lambda^sub max^ = 613 nm), while the reverse emitted ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

NEW LIQUID FORMULATION OF FOLLITROPIN ALFA (RECOMBINANT-HFSH): BIOEQUIVALENCE TO THE CURRENT FREEZE-DRIED FORMULATION
; ...non-compartmental analysis. AUQ^sub last^ and C^sub max^ were calculated, log-transformed and analysed using ANOVA/ANCOVA; t^sub max^ was also computed. Average bioequivalence...subjects were evaluable. Means of C^sub max^ and AUClast were similar (C^sub max...
PET Uptake as Predictive Biomarker in Cervical Cancer
; ...maximized standard uptake value [SUV^sub max^]) of ^sup 18^F-FDG as evaluated by PET...chemoradiation, or palliation. The mean SUV^sub max^ was 11.4, with a wide range of 1-50.4...was found between tumor volume and SUV^sub max^ or between type of tumor histology and...
Using Trypsin & Soybean Trypsin Inhibitor To Teach Principles of Enzyme Kinetics
; ...that follow Michaelis-Menten kinetics, V^sub max^ and K^sub m^ describe key parameters of their function. The maximum velocity, V^sub max^, is defined as the rate of product formation...enzyme is saturated with substrate. At V^sub max^, enzyme-substrate binding is not limiting...
Analytical Model to Predict Nonlinear Flexural Behavior of Corroded Reinforced Concrete Beams. Paper by Tamer El Maaddawy, Khaled Soudki, and Timothy Topper: Discussion by Peter H. Bischoff/AUTHORS' CLOSURE
; ...stress at the end of an element as Δf^sub max^ = 4τs^sub m^/2d^sub b^ for a constant...in bar stress at first cracking Δf^sub max,cr^ = 4τ^sub o^s^sub m^/2d^sub b...The expression τ^sub o^ = Δf^sub max,cr^d^sub b^/2s^sub m^ then represents...
Analytical Model to Predict Nonlinear Flexural Behavior of Corroded Reinforced Concrete Beams. Paper by Tamer El Maaddawy, Khaled Soudki, and Timothy Topper/AUTHORS' CLOSURE
; ...stress at the end of an element as Δf^sub max^ = 4τs^sub m^/2d^sub b^ for a constant...in bar stress at first cracking Δf^sub max,cr^ = 4τ^sub o^s^sub m^/2db occurs...The expression τ^sub o^ = Δf^sub max,cr^d^sub b^/2s^sub m^ then represents...
A Spectral Technique for Estimating Turbulent Stress, Scalar Flux Magnitude, and Eddy Viscosity in the Ocean Boundary Layer under Pack Ice
; ...universal" function of nondimensional wavenumber k/k^sub max^, where k^sub max^ is the wavenumber at the peak in the area-preserving...measured Reynolds stress magnitude and wavenumber by k^sub max^. In the outer boundary layer, normalized spectra...
PET and Survival After Lung Resection
; ...months (range, 16-18 months). The median maximal SUV (SUV^sub max^) was 9. For patients in whom SUV^sub max^ was >9, 2-year survival was at 68%, whereas for those in whom SUV^sub max^ was <9 this figure was 96%. Analysis showed that only...
Spectral Tuning of Shortwave-sensitive Visual Pigments in Vertebrates[dagger]
; ...shows some of the largest shifts in λ^sub max^, with values ranging in different species...ancestral pigment most probably had a λ^sub max^ in the UV and that shifts between violet...characteristic peak of maximal absorbance (λ^sub max^), the precise location of this ...
Acclimation of rice photosynthesis to irradiance under field conditions
; ...photosynthetic carbon assimilation rates (P^sub max^), Rubisco, and pigment content in mature...Rubisco amount, chlorophyll a/b, and P^sub max^ all declined from the top to the base...artificial shading experiment, acclimation of P^sub max ^ (measured at 350 (mu)L L^sup -1^ CO2...
The Effect of Irrigation on Regional Temperatures: A Spatial and Temporal Analysis of Trends in California, 1934-2002
; ...maximum temperatures (T^sub min^ and T^sub max^) in California was estimated using historical...of irrigation on June-August average T^sub max^, with no significant effects on T^sub min^ (p > 0.3). The mean estimate for T^sub max^ was a substantial 5.0C cooling for 100...