Characterization of Amplifiable, Circulating RNA in Plasma and Its Potential as a Tool for Cancer Diagnostics

From: Clinical Chemistry | Date: March 1, 2004| Author: Bigbee, William L; Et al; Kelly, Lori; Raja, Siva; El-Hefnawy, Talal | Copyright information

Background: Several recent reports have described the detection of circulating, cancer-related RNA molecules in serum or plasma from cancer patients, but little is known about the biology of this extracellular RNA. We aimed to determine how RNA is protected against degradation in serum, to optimize RNA isolation from large volumes of serum, and to test our optimized assays for serum-based cancer detection.

Methods: We used quantitative reverse transcription-PCR (QRT-PCR) analysis to in...

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