Simple Quantitative HPLC Method for Measuring Physiologic Amino Acids in Cerebrospinal Fluid without Pretreatment From: Clinical Chemistry | Date: March 1, 2004| Author: Sethuraman, Rama; Lee, Tat Leang; Tachibana, Shinro | Copyright American Association for Clinical Chemistry Mar 2004. Provided by ProQuest LLC.Copyright information

Because the cerebral spinal fluid (CSF) bathes the whole central nervous system, its contents reflect changes occurring in the central neurons. Quantitative analysis of amino acids present in CSF has been helpful in improving our understanding of disease conditions associated with pain (1-3). Amino acids in CSF can be determined either by postcolumn derivatization with ninhydrin or o-phthalaldehyde (OPA) by commercially available amino acid analyzers or by precolumn derivatization with differ...

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