Charged Polymers Modulate Retrovirus Transduction via Membrane Charge Neutralization and Virus Aggregation

From: Biophysical Journal | Date: February 1, 2004| Author: Morgan, Jeffrey R; Rosinski, Matthew; Yarmush, Martin L; Davis, Howard E | Copyright information

ABSTRACT

The specific mechanisms of charged polymer modulation of retrovirus transduction were analyzed by characterizing their effects on virus transport and adsorption. From a standard colloidal perspective two mechanisms, charge shielding and virus aggregation, can potentially account for the experimentally observed changes in adsorption behavior and biophysical parameters due to charged polymers. Experimental testing revealed that both mechanisms could be at work depending on the c...

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