Visible Light Irradiation of Ethidium Bromide-stained Interphase Nuclei Causes DNA-Protein Linking and Structural Stabilization of Nucleoprotein Complexes6

From: Photochemistry and Photobiology | Date: December 1, 2003| Author: | Copyright information

ABSTRACT

Fixation of DNA and proteins in the isolated rat hepatocyte nuclei stained with ethidium bromide and irradiated with visible light was analyzed in this study. It was shown that irradiation results in the following modifications of higher- level nucleoprotein complexes of interphase chromatin: (1) the complexes acquire resistance to decondensing treatments, which may be indicative of the formation of links between proteins or proteins and DNA in the chromatin; (2) the linking rate for both DNA and proteins is dose dependent; (3) the irradiation induces intermolecular link formation ...

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