Time-resolved Fluorescence Resonance Energy Transfer Assay for Point-of-Care Testing of Urinary Albumin

From: Clinical Chemistry | Date: July 1, 2003| Author: | Copyright information

Background: Microalbuminuria is an established early marker of diabetic nephropathy and an important cardiovascular risk factor in diabetes and hypertension. We aimed to develop a rapid point-of-care assay for the measurement of urine albumin.

Methods: The competitive homogeneous assay used an albumin-specific monoclonal antibody labeled with a stable fluorescent europium chelate as donor and an albumin labeled with cyanine 5 (Cy5) as acceptor. The assay was performed at room temperature in single microtitration wells that contained all the required dryform reagents. The close proximity ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

Energy Transfer Among Dyes on Particulate Solids[dagger]
; ...emphasis on radiative and nonradiative energy transfer from Pheo to MB. Although pure MB...experimental data is developed, through which energy transfer efficiencies can be calculated for...assuring no dye aggregation, nonradiative energy transfer efficiencies reach a maximum value...
A Flexible Approach to the Calculation of Resonance Energy Transfer Efficiency between Multiple Donors and Acceptors in Complex Geometries
; ABSTRACT Resonance energy transfer provides a practical way to...relationship between the efficiency of energy transfer and the distance between sites...we demonstrate how resonance energy transfer can be used to measure distances...
Energy transfer within the small-scale oceanic internal wave spectrum
; ...experiments are conducted to examine energy transfer within the small-scale portion of...oceanic internal waves. The rate of energy transfer in the experiments is a significant...wavenumber-local interactions dominate the energy transfer rather than scale-separated ones...
Energy Transfer Partners to acquire Canyon Gas system
; Energy Transfer Partners LP, the Dallas-based owner...Uinta basins in Colorado and Utah, Energy Transfer said Friday in a statement. Metalmark...where natural gas output is rising. Energy Transfer Partners in November bought Transwestern...
Energy transfer capitalizes on being in the right place at the right time.(A Midstream Giant)(Company overview)
; ...eye on the big picture to create Energy Transfer Partners, L.P., an increasingly...timing and luck played key roles in Energy Transfer's development. Good timing and good luck for Energy Transfer, bad timing and bad luck for some...
Effective lattice behavior of fluorescence energy transfer at lamellar macromolecular interfaces
; ABSTRACT Fluorescence energy transfer between donors and acceptors...e., critical radius of the energy transfer). Furthermore, a "discrete...apparent dimensionality of the energy transfer with increasing acceptor concentration...
Contactless energy transfer: Mobile power on the go.
; ...interest among engineers is contactless energy transfer for powering and controlling motors...phenomenon in the U.S. With contactless energy transfer, a non-contact radio frequency system...a production process. Contactless energy transfer can be used overhead in ...
Energy transfer, heritage deal adds natural gas operations.(Tulsa, Oklahoma)(U.S. Propane LLC acquisition by affiliates of Energy Transfer )
; ...between Heritage Propane Partners and Energy Transfer Co. allows affiliates of Energy Transfer to buy U.S. Propane LP--Heritage's...Propane LLC. Meanwhile, Heritage will add Energy Transfer's natural gas midstream operations...
LP/gas stock index.(AmeriGas Inc.)(Energy Transfer Co.)(Ferrellgas Inc.)(Statistical table)
; ...10/13/06) AmeriGas APU June $30.46 Energy Transfer ETP May 46.38 Ferrellgas FGP July...Tax Deferral AmeriGas $2.32 7.6% 80% Energy Transfer 3.00 6.5 70 Ferrellgas 2.00 9.3 90...Outstanding (millions) AmeriGas 74% 35% 56.8 Energy Transfer 50 26 110.7 Ferrellgas 79 43 60.8...
LP/Gas stock index.(AmeriGas Inc.)(Energy Transfer Co.)(Ferrellgas Inc.)(Statistical table)
; ...9/15/06) AmeriGas APU June $30.20 Energy Transfer ETP May 46.70 Ferrellgas FGP Apr...Yield Deferral AmeriGas $2.32 7.7% 80% Energy Transfer 2.55 5.5 70 Ferrellgas 2.00 8.7 90...Cap (millions) AmeriGas 74% 35% 56.8 Energy Transfer 50 26 110.7 Ferrellgas 75 41 60.4...