Inductively coupled plasma mass spectrometric analysis of calcium isotopes in human serum: A low-sample-volume acid-equilibration method

From: Clinical Chemistry | Date: December 1, 2003| Author: Abrams, Steven A; Griffin, Ian J; Kriseman, Yana L; Liang, Lily K; Chen, Zhensheng | Copyright information

Background: Analytical methods for measuring the calcium isotope distribution in enriched human serum samples that use low blood volumes, simple preparation methods, and rapid analysis are important in clinical studies of calcium kinetics. Previously, sample preparation by oxalate precipitation typically required 500 µL of serum. This method was time-consuming, and the blood volume required was limiting in circumstances when only a small amount of serum could be obtained.

Methods: Ser...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and journal articles from HighBeam Research

Inductively coupled plasma mass spectrometric analysis of calcium isotopes in human serum: A low-sample-volume acid-equilibration method
Clinical Chemistry ; Background: Analytical methods for measuring the calcium isotope distribution in enriched human serum samples that use low blood volumes, simple preparation methods, and rapid analysis are important in clinical studies of calcium kinetics. Previously, sample preparation by oxalate precipitation
Natural Calcium Isotopic Composition of Urine as a Marker of Bone Mineral Balance
Clinical Chemistry ; Background: We investigated whether changes in the natural isotopic composition of calcium in human urine track changes in net bone mineral balance, as predicted by a model of calcium isotopic behavior in vertebrates. If so, isotopic analysis of natural urine or blood calcium could be used to
Measuring What Isn't There
Clinical Chemistry ; In the rush to develop "peaceful uses of the atom" in the years following testing of the H-bomb, much interesting work was done with radioactive calcium isotopes (^sup 45^Ca and ^sup 47^Ca). Perhaps most important was the finding that the relatively huge quantities of calcium contained in mature
Science: Update
The Independent - London ; THE COUNCIL of Europe is to vote today on whether to impose a moratorium on clinical trials of xenotransplantation, in which animal organs are transplanted into humans. The vote, which is expected to halt plans for trials, would be a political rather than a scientific decision, and probably not
Let there be aluminum-42: experiment creates surprise isotope.(This Week)
Science News ; Physicists have created the heaviest isotope yet of magnesium, but in their experiments an unexpected isotope of aluminum also showed up. The findings could help astrophysicists understand occasional X-my emissions from neutron stars that are growing in mass. The 7-day-long experiment took place at
Modification and validation of a single-isotope radiocalcium absorption test
The Journal of Nuclear Medicine ; This study was done to establish and allow for the influence of body weight on plasma radioactivity after administering radiocalcium to measure calcium absorption. Methods: We administered 5 (mu)Ci ^sup 45^Ca in 20 mg of calcium carrier in 250 ml distilled water to 103 premenopausal volunteers over
Controlled high meat diets do not affect calcium retention or indices of bone status in healthy postmenopausal women
The Journal of Nutrition ; Controlled High Meat Diets Do Not Affect Calcium Retention or Indices of Bone Status in Healthy Postmenopausal Women1,2 ABSTRACT Calcium balance is decreased by an increased intake of purified proteins, although the effects of common dietary sources of protein (like meat) on calcium economy remain
Quantifying human calcium absorption using pharmacokinetic methods
The Journal of Nutrition ; Quantifying Human Calcium Absorption Using Pharmacokinetic Methods1 ABSTRACT Calcium absorption was measured simultaneously by tracer and pharmacokinetic methods in 12 men. The purpose of the study was to calibrate the area under the curve (AUC) for absorptive calcemia to permit comparison or
Columbia shuttle blasts off to perform tough medical tests in space.(Originated from Knight-Ridder Newspapers)
Knight Ridder/Tribune News Service ; CAPE CANAVERAL, Fla. _ Bring on the needles and probes, the isotopes and electrodes. The seven astronauts aboard shuttle Columbia blasted safely into orbit on Thursday, generally the most dangerous and unpleasant phase of any space mission. But now, they face new challenges and unpleasantries _ a
Calcium Bioavailability of Calcium Carbonate Fortified Soymilk Is Equivalent to Cow's Milk in Young Women1,2
The Journal of Nutrition ; ABSTRACT Calcium (Ca)-fortified soymilk has gained popularity in the United States. Tricalcium phosphate (TCP)-fortified soymilk was shown to have a lower Ca bioavailability than cow's milk in men. However, the most popular soymilk in the U.S. is fortified with Ca carbonate (CC) and has not been