Immortalization of chinchilla middle ear epithelial cells by adenovirus 12-simian virus 40 hybrid virus

The Annals of Otology, Rhinology & Laryngology | October 1, 1999| | Copyright

In order to study the cellular and molecular mechanisms of the pathogenesis of otitis media, a chinchilla middle ear epithelial cell line (CMEE-1) with differentiated cell characteristics was established by infection of a primary culture with the adenovirus 12simian virus 40 (Adl2-SV40) hybrid. This cell line has been in continuous culture for 42 passages, whereas the parent cells underwent senescence and died at the 8th passage. The cell line also retains epithelial morphology and expresses cytokeratin polypeptides 4, 7, and 18, characteristic markers for epithelia. In Western blots of cell ...

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