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Two-photon fluorescence microscopy observation of shape changes at the phase transition in phospholipid giant unilamellar vesicles
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ABSTRACT Using the sectioning effect of the two-photon fluorescence microscope, we studied the behavior of phospholipid giant unilamellar vesicles (GUVs) composed of pure diacylphosphatidylcholine phospholipids during the gel-to-liquid crystalline phase transition. We used the well-characterized excitation generalized polarization function (GP^sub ex^,) of 6-dodecanoyl-2-dimethylamine-naphthalene (LAURDAN), which is sensitive to the changes in water content in the lipid vesicles, to monitor the phase transition in the GUVs. Even though the vesicles do not show temperature hysteresis at the ...
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Direct Visualization of Asymmetric Behavior in Supported Lipid Bilayers at the Gel-Fluid Phase Transition
; ...microscopy to examine the gel-fluid phase transition behavior in supported phospholipid...2-dipalmitoyl-sn-glycero-3-phosphocholine. The primary gel-fluid phase transition at T^sub m^ occurs through development...reveal the presence of a secondary phase transition in all three ...
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Response to selection for the timing of vegetative phase transition in a maize population. (Crop Breeding, Genetics & Cytology).(Statistical Data Included)
; ...Bongard-Pierce et al., 1996). The most apparent phase transition related trait is the presence of epicuticular...vegetative phase change. Vegetative phase transition occurs at a predictable time in shoot...suggests that the timing of vegetative phase transition has a genetic basis ...
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From fireball to galaxies: making late waves.
; ...on a cosmological scale require a phase transition -- a change in the fabric of space...collaborators suggest that such a phase transition occurred a million or so years after...thought, Schramm says. Allowing the phase transition to be late opens up all sorts of new...
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Universe in a drop; cosmology in the liquid laboratory. (phase transition research applied to study of universe evolution)
; ...superfluid helium is an example of a phase transition. Assuming that the universe was once...issue concerns what happens during a phase transition. For example, when water freezes slowly...College in London suggested that a rapid phase transition just a tiny fraction of a second ...
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Electrochemical and PM-IRRAS Studies of the Effect of Cholesterol on the Structure of a DMPC Bilayer Supported at an Au (111) Electrode Surface, Part 1: Properties of the Acyl Chains
; ...of cholesterol is increased, the main phase transition region becomes broad, and the main phase transition temperature decreases correspondingly...appears at temperatures above the main phase transition temperature at high concentrations...
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Binding of adriamycin to liposomes as a probe for membrane lateral organization
; ...was seen throughout the main phase transition region as well as in the...below, above, and at the main phase transition temperature is interpreted...involved in the phospholipid main phase transition. This is the first observation...
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Phase Behavior and Nanoscale Structure of Phospholipid Membranes Incorporated with Acylated C^sub 14^-Peptides
; ...concentration-dependent downshift of both the main phase transition and the pretransition. In addition, the main phase transition peak is significantly broadened...enhanced in the vicinity of the main phase transition, where the lipid membrane...
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Symmetry and the devil.
; ...simplified interpretation of phase transition findings. The authors show that attention to the details of phase transition analysis, as well as consideration...one type of result from the phase transition literature: the abrupt transition...
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Calorimetric and spectroscopic studies of the thermotropic phase behavior of lipid bilayer model membranes composed of a homologous series of linear saturated phosphatidylserines
; ...calorimetrically detectable phase transition that is fully reversible, highly...Ld-to-lamellar liquid crystalline (Lj phase transition. However, after low temperature incubation, the L,/La phase transition of dilauroyl phosphatidylserine...
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Lipid phase transition temperatures
; Lipid phase transition temperatures Michel, N.,A.-S...and B. Pucci, Determination of Phase Transition Temperatures of Lipids by Light...supramolecular systems induced by the phase transition was correlated with the refraction...
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