Activation of the calcium-regulated thin filament by myosin strong binding From: Biophysical Journal | Date: October 1, 2003| Author: Gorga, Joseph A; Fishbaugher, David E; VanBuren, Peter | Copyright Biophysical Society Oct 2003. Provided by ProQuest LLC.Copyright information

ABSTRACT The current study was undertaken to investigate the relative contribution of calcium and myosin binding to thin filament activation. Using the in vitro motility assay, myosin strong binding to the thin filament was controlled by three mechanisms: 1), varying the myosin concentration of the motility surface, and adding either 2), inorganic phosphate (Pi) or 3), adenosine diphosphate (ADP) to the motility solutions. At saturating myosin conditions, Pi had no effect on thin filament mot...

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