Pentacoordinate hemin derivatives in sodium dodecyl sulfate micelles: Model systems for the assignment of the fifth ligand in ferric heme proteins

From: Biophysical Journal | Date: August 1, 1999| Author: | Copyright information

ABSTRACT Ferric iron protoporhyrin IX derivatives in SDS micelles have been investigated by means of visible absorption, resonance Raman, and XANES spectroscopies to establish specific correlations between the marker bands of the pentacoordinate derivatives obtained from the three different techniques. Hydroxyl and 1,2-dimethyl imidazole coordinated hemins display the typical spectroscopic marker bands of a pentacoordinate high-spin ferric iron derivative in both Raman and XANES spectra. In turn, the optical absorption spectra of these two derivatives are very different. This difference is in ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

Time-Resolved Visible and Infrared Study of the Cyano Complexes of Myoglobin and of Hemoglobin I from Lucina pectinata
; ...dynamics of the ferric CN complexes of the heme proteins Myoglobin and Hemoglobin I from the...on the same timescale. INTRODUCTION Heme proteins are widely studied as model systems...O2, NO, or CO, which bind to ferrous heme proteins (Fe^sup II^), hydrogen sulphide binds...
A study of vibrational relaxation of B-state carbon monoxide in the heme pocket of photolyzed carboxymyoglobin
; ...CO) dissociation and rebinding in heme proteins. Investigation of CO relaxation in heme proteins can provide vital information concerning...heme compounds (Hill et al., 1995) and heme proteins (Hill et al., 1994; Owrutsky et al...
Electric-Field-Induced Redox Potential Shifts of Tetraheme Cytochromes c^sub 3^ Immobilized on Self-Assembled Monolayers: Surface-Enhanced Resonance Raman Spectroscopy and Simulation Studies
; ...INTRODUCTION Electron transferring heme proteins are constituents of a large variety...determine the intrinsic redox potential of heme proteins include the axial ligation pattern...Because biological redox reactions of heme proteins take place at or in membranes or in...
Viscosity-Dependent Protein Dynamics
; ...viscosity at room temperature in four heme proteins: hemoglobin, myoglobin, a myoglobin...relationship between structure and dynamics in heme proteins continues to serve as an elegant example...the viscosity-dependent dynamics of heme proteins have continued to support the strong...
Iron normal mode dynamics in (nitrosyl)iron(II)tetraphenylporphyrin from X-ray nuclear resonance data
; ...heme dynamics in myoglobin and other heme proteins. Nuclear resonance vibrational spectroscopy...dynamics in the biological functioning of heme proteins. INTRODUCTION This work was supported...R. 2000. Resonance Raman spectra of heme proteins and model compounds. In The Porphyrin...
^sup 15^N-^sup 1^H Residual Dipolar Coupling Analysis of Native and Alkaline-K79A Saccharomyces cerevisiae Cytochrome c
; ...Tjandra, 1997; Tolman et al., 1997). Heme proteins possess molecular magnetic anisotropy...Bentrop et al., 1997). In diamagnetic heme proteins, the magnetic anisotropy is small and...constraints. In paramagnetic low-spin heme proteins, the RDCs are in the range - 1.5 to...
Noninvasive Auto-Photoreduction Used as a Tool for Studying Structural Changes in Heme-Copper Oxidases by FTIR Spectroscopy
; ...auto-photoreduction method should be universally applicable to heme proteins. INTRODUCTION Cytochrome oxidases are the terminal enzymes...spectral region of 1100-1650 cm^sup -1^. As reported earlier, heme proteins, in which the central iron is in the ferric state, can be...
Examining mechanisms of lipid oxidation and its inhibition
; ...understanding the pathway by which heme proteins promote lipid oxidation is that several...variation in the ability of different heme proteins from fish, poultry, and bovine animals...understanding of mechanisms by which heme proteins promote lipid oxidation. Another area...
Influence of static and dynamic disorder in the visible and infrared absorption spectra of carbonmonoxy horseradish peroxidase
; ...dynamics and protein function has received much attention in heme proteins. Kinetic measurements of CO recombination in myoglobin after...myoglobin have been extensively studied, a comparison with other heme proteins is one way to examine how protein dynamics vary, how physical...
Computer charts path of diffusing molecules. (changes in sequence alter entrance rate)
; ...because the sites where oxygen or carbon monoxide bind to heme proteins lie deeply buried and seem inaccessible. The simulations...developing blood substitutes and understanding oxygen transport by heme proteins, biochemist John S. Olson at Rice University in Houston uses...