Detection of anti-Yt antibodies using a sensitive and specific enzyme-linked immunosorbent assay

From: British Journal of Biomedical Science | Date: January 1, 1999| Author: | Copyright information

Abstract: A specific, sensitive and semi-quantitative enzyme-linked immunosorbent assay (ELISA) is described to detect anti-Yt^sup a^ antibodies in human serum. Recombinant acetylcholinesterase (AChE E.C.3.1.1.7) was employed as the coating antigen in the microtitre plate and horseradish peroxidase (HRP)-conjugated specific antibody (IgG) was used as the secondary antibody. The method developed showed excellent sensitivity, detecting a titre > 1 in 600 000 (3.5 ng/mL mouse IgG protein) for mouse monoclonal (mMAb) antiAChE antibody. No cross-reaction was seen with other common blood group ...

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