Comparative evaluation of protective antigen produced from Bacillus anthracis & Escherichia coli

From: Indian Journal of Medical Research | Date: March 1, 2003| Author: | Copyright information

Background & objective: Anthrax has been reported from almost every country and India is endemic for this disease. There is considerable under reporting of the disease because of lack of microbiological facilities and diagnostic reagents. In India only conventional methods which have limitations, are being used to diagnose the disease. Hence the aim of this study was to isolate and purify protective antigen (PA) using different protocols and to use this PA for detection of anti-PA antibodies from sera samples.

Methods: Protective antigen was isolated and purified from the Sterne strain of ...

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