Continuous cell lines from the common white, Pieris rapae crucivora Boisduval

From: In Vitro Cellular & Developmental Biology | Date: March 1, 2003| Author: | Copyright information

SUMMARY

Three continuous cell lines, NIAS-PRC-819A, NIAS-PRC-819B, and NIAS-PRC-819C, were established from the pupal ovaries of the common white, Pieris rapae crucivora Boisduval (Insecta, Lepidoptera, Pieridae). The primary culture was initiated as expiant cultures with ovariole fragments in MGM-464 medium supplemented with 20% fetal bovine serum at 25[degrees] C. About 6 mo after the culture was set up, the first subculture was prepared. Thereafter, cells were subcultured with decreasing passage intervals, resulting in a cell population that multiplied continuously. The karyotypes of these ...

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