Isolation and culture of the three vascular cell types from a small vein biopsy sample

From: In Vitro Cellular & Developmental Biology | Date: March 1, 2003| Author: | Copyright information

SUMMARY

The availability of small-diameter blood vessels remains a significant problem in vascular reconstruction. In small-diameter blood vessels, synthetic grafts resulted in low patency; the addition of endothelial cells (EC) has clearly improved this parameter, thereby proving the important contribution of the cellular component to the functionality of any construct. Because the optimal source of cells should be autologous, the adaptation of existing methods for the isolation of all the vascular cell types present in a single and small biopsy sample, thus reducing patient's morbidity, is ...

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