Enhanced myogenic differentiation by extracellular matrix is regulated at the early stages of myogenesis

In Vitro Cellular & Developmental Biology | March 1, 2003| | Copyright

SUMMARY

Myogenic cell lines have been used extensively in the study of skeletal muscle development, regeneration, and homeostasis. To induce myogenic differentiation, culture media composed of a wide variety of growth factors and other additives have been used. Because the diversity in these components may modulate the differentiation process differentially, we describe a differentiation protocol that does not require the introduction of any factors to the differentiation media (DM) other than those present in the growth media. By culturing C2C12 skeletal myocytes on a coating of diluted ...

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