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Phosphorylation of the potyvirus capsid protein by protein kinase CK2 and its relevance for virus infection(W)
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We reported previously that the capsid protein (CP) of Potato virus A (PVA) is phosphorylated both in virus-infected plants and in vitro. In this study, an enzyme that phosphorylates PVA CP was identified as the protein kinase CK2. The [alpha]-catalytic subunit of CK2 (CK2a) was purified from tob
acco and characterized using in-gel kinase assays and liquid chromatographytandem mass spectrometry. The tobacco CK2a gene was cloned and expressed in bacterial cells. Specific antibodies were raised against the recombinant enzyme and used to demonstrate the colocalization of PVA CP and CK2t* in ...
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Transcript from: NPR Talk of the Nation Science Friday
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Transcript from: NPR All Things Considered
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Transcript from: NPR All Things Considered
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Transcript from: NPR All Things Considered
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Transcript from: CBS Morning News
; ...combining with the powerful nor`easter creating what some experts call a perfect storm. Heavy rain has battered the region for days leading to widespread flooding. So far, Virginia has bared the brunt of the nasty storm. The governor`s even declared a...
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Transcript from: Congressional Testimony
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Transcript from: Regulatory Intelligence Data
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Transcript from: Washington Transcript Service
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Transcript from: Washington Transcript Service
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Transcript from: NPR Weekend Edition - Saturday
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