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Separation of water-soluble proteins from cereals by high-performance capillary electrophoresis (HPCE)
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ABSTRACT
Most research concerning grain proteins has concentrated on the gluten storage proteins. The albumins and globulins are the water- and salt-soluble proteins that contain biologically active enzymes and enzyme inhibitors. A free-zone capillary electrophoresis method was developed to separate these proteins. Optimization included sample extraction method, capillary temperature, buffer composition, and additives. The optimal conditions for separation of these proteins was 50 [mu]m i.d. X 27 cm (20 cm to detector) capillary at 10 kV (with a 0.17 min ramp-up time) and 25[degrees]C. The ...
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Protein storage bodies and vacuoles
; ...livestock. Consequently, the biochemistry of storage proteins and the cellular and physiological mechanisms...processes involved in the accumulation of storage proteins. Storage proteins accumulate primarily in the protein storage...
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Zeolin. A New Recombinant Storage Protein Constructed Using Maize [gamma]-Zein and Bean Phaseolin1
; The major seed storage proteins of maize (Zea mays) and bean...another protein. Because the storage proteins of cereals and legumes nutritionally...balanced and highly stable chimeric storage proteins. Seeds accumulate very large...
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MAIGO2 Is Involved in Exit of Seed Storage Proteins from the Endoplasmic Reticulum in Arabidopsis thaliana(W)(OA)
; Seed storage proteins are synthesized on the endoplasmic...accumulated the precursors of two major storage proteins, 2S albumin and 12S globulin...accumulate large quantities of storage proteins, such as globulins and albumins...
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The Proteolytic Processing of Seed Storage Proteins in Arabidopsis Embryo Cells Starts in the Multivesicular Bodies(W)
; ...have investigated the transport of storage proteins, their processing proteases, and the...and subcellular fractionation. The storage proteins and their processing proteases are...the compartmentalized processing of storage proteins in the MVBs may allow for the sequential...
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Arabidopsis Vacuolar Sorting Mutants (green fluorescent seed) Can Be Identified Efficiently by Secretion of Vacuole-Targeted Green Fluorescent Protein in Their Seeds(W)(OA)
; ...function in vacuolar sorting of seed storage proteins: a vacuolar sorting receptor, VSR1...all of which caused missorting of storage proteins. We mapped gfs1 to VSR1, gfs2 to KAM2...mechanisms underlying vacuolar sorting of storage proteins. INTRODUCTION Higher plants synthesize...
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Endogenous Action of Cysteine Endopeptidase and Three Carboxypeptidases on Triticale Prolamins
; ...endopeptidases. Cereal Chem. 85(3):366-371 Storage proteins of cereal grains can be divided into...of bases (Osborne 1907). The main storage proteins of wheat or rye are prolamins, which...The hydrolysis of wheat kernels storage proteins is the most intensive on days 2 to...
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Molecular Characterization of a Novel Methionine-Rich [delta]-Kafirin Seed Storage Protein Gene in Sorghum (Sorghum bicolor L.)
; ...kafirin proteins with other seed storage proteins from maize, rice, and Brazil nut revealed...for protein synthesis. Cereal seed storage proteins also play an important role in food...Shewry and Halford 2002). Cereal seed storage proteins are synthesized at high levels in...
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Transgenic Soybean Seeds for the Elevated Production of Foreign Proteins
; ...comprised of the two major storage proteins: 7S conglycinin and 11S glycinin...down-regulate the 7S and 11S storage proteins, both individually and in...Along with the targeting of storage proteins, RNAi constructs specific...
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Genomics analysis of genes expressed in maize endosperma identifies novel seed proteins and clarifies patterns of zein gene expression
; ...endosperm. INTRODUCTION Genes encoding storage proteins are highly expressed in developing...proteins and the mechanisms by which storage proteins are synthesized (Herman and Larkins...Shewry and Casey, 1999). Traditionally, storage proteins were identified on the basis of their...
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Redundant proteolytic mechanisms process seed storage proteins in the absence of seed-type members of the vacuolar processing enzyme family of cysteine proteases
; ...translational proteolysis of seed storage proteins in the protein storage vacuole of...temporal expression patterns with seed storage proteins. From these results, we conclude that...proteolytic enzymes also can process storage proteins into chains capable of stable accumulation...
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