Evaluation of novel precast SDS-PAGE gels for separation of sorghum proteins

From: Cereal Chemistry | Date: September 1, 2003| Author: | Copyright information

ABSTRACT

Precast polyacrylamide gels using novel buffer chemistry for enhanced resolution and shelf life stability of the gels were evaluated for separating sorghum proteins. Two gels with different acrylamide concentrations, 12 and 4-12%, were tested with two different buffer systems. Gels were evaluated for separation resolution, as well as protein solubility problems such as streaking. High-resolution separations were obtained for all the major classes of kernel proteins using these gels. Run times were typically 45-60 min, producing relatively rapid separations. Resolution was ...

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