Activity spectra of Bacillus thuringiensis delta-endotoxins against eight insect cell lines

From: In Vitro Cellular & Developmental Biology | Date: May 1, 1999| Author: | Copyright information

SUMMARY

Eight continuous insect cell lines were tested for susceptibility to the 6-endotoxins of several lepidopteran-active strains and cloned-gene products of Bacillus thuringiensis. The assays were performed on cells suspended in agarose gel, which allowed the toxins activated at pH 10.5 to be applied directly in a high-pH buffer without causing solvent toxicity to the cells. The responses of the cell lines to the various toxins produced activity spectra that were used to identify functionally similar and dissimilar toxin proteins.

IPRI-CF-1 and FPMI-MS-5, derived from neonate larvae of ...

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