Application of rapid-cycle real-time polymerase chain reaction for diagnostic testing in the clinical microbiology laboratory

From: Archives of Pathology & Laboratory Medicine | Date: September 1, 2003| Author: | Copyright information

* Context.-Rapid-cycle real-time polymerase chain reaction (PCR) technology combines rapid thermocycling with real-time fluorescent probe detection of amplified target nucleic acids.

Objectives.-To review and compare the method of rapid-cycle real-time PCR to conventional PCR methods. To describe the application of rapid-cycle real-time PCR for diagnostic testing in the microbiology laboratory.

Data Selection.-Information is presented from published literature as well as from personal experience at the Mayo Clinical Microbiology Laboratory (Rochester, Minn).

Conclusions.-Compared to ...

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