Probing conformational changes of gramicidin ion channels by single-molecule patch-clamp fluorescence microscopy From: Biophysical Journal | Date: September 1, 2003| Author: | Copyright Biophysical Society Sep 2003. Provided by ProQuest LLC.Copyright information

ABSTRACT

Complex conformational changes influence and regulate the dynamics of ion channels. Such conformational changes are stochastic and often inhomogeneous, which makes it extremely difficult, if not impossible, to characterize them by ensemble-averaged experiments or by single-channel recordings of the electric current that report the open-closed events but do not specifically probe the associated conformational changes. Here, we report our studies on ion channel conformational changes using a new approach, patch-clamp fluorescence microscopy, which simultaneously combines ...

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