Diel cycles of DNA damage and repair in eggs and larvae of northern archovy, Engraulis mordax, exposed to ultraviolet radiation

From: Photochemistry and Photobiology | Date: January 1, 1999| Author: | Copyright information

ABSTRACT

Damage from UVB radiation (280-320 nm) in the form of cyclobutane pyrimidine dimers (CPD) in DNA and the capacity for their repair were measured in newly spawned eggs and yolk-sac larvae of northern anchovy, Engraulis mordax, exposed to natural diel cycles of sunlight. The CPD were measured by a newly developed chemiluminescent immunoblot assay capable of measuring CPD in samples as small as 50 ng DNA. Eggs and yolk-sac larvae exposed to full irradiance levels died. At lower dose rates, equivalent to deeper more natural locations in the water column, there was a diel cycle of dimer ...

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