Solution x-ray scattering-based estimation of electron cryomicroscopy imaging parameters for reconstruction of virus particles

From: Biophysical Journal | Date: April 1, 1999| Author: | Copyright information

ABSTRACT Structure factor amplitudes and phases can be computed directly from electron cryomicroscopy images. Inherent aberrations of the electromagnetic lenses and other instrumental factors affect the structure factors, however, resulting in decreased accuracy in the determined three-dimensiona l reconstruction. In contrast, solution x-ray scattering provides absolute and accurate measurement of spherically averaged structure factor amplitudes of particles in solution but does not provide information on the phases. In the present study, we explore the merits of using solution x-ray ...

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