Hydrodynamic properties of human erythrocyte band 3 solubilized in reduced triton X-100

From: Biophysical Journal | Date: April 1, 1999| Author: | Copyright information

ABSTRACT The oligomeric state and function of band 3, purified by sulfhydryl affinity chromatography in reduced Triton X-100, was investigated. Size exclusion high-performance liquid chromatography showed that a homogeneous population of band 3 dimers could be purified from whole erythrocyte memb ranes. The elution profile of band 3 purified from membranes that had been stripped of its cytoskeleton before solubilization was a broad single peak describing a heterogeneous population of oligomers with a mean Stokes radius of 100 Angstrom. Sedimentation velocity ultracentrifugation analysis ...

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