Tetraethylammonium block of the BNC1 channel

From: Biophysical Journal | Date: March 1, 1999| Author: | Copyright information

ABSTRACT The brain Na+ channel-1 (BNC1, also known as MDEG1 or ASIC2) is a member of the DEG/ENaC cation channel family. Mutation of a specific residue (Gly430) that lies N-terminal to the second membrane-spanning domain activates BNC1 and converts it from a Na+-selective channel to one permeable to both Na+ and K+. Because all K+ channels are blocked by tetraethylammonium (TEA), we asked if TEA would inhibit BNC1 with a mutation at residue 430. External TEA blocked BNC1 when residue 430 was a Val or a Thr. Block was steeply voltage-dependent and was reduced when current was outward, ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

Sodium fluxes through nonselective cation channels in the plasma membrane of protoplasts from Arabidopsis roots
; Sodium Fluxes through Nonselective Cation Channels in the Plasma Membrane of Protoplasts...sup +^ currents through nonselective cation channels (NSCCs) in protoplasts derived from...root protoplasts were mediated by cation channels that were insensitive to externally...
Estimating the Sensitivity of Mechanosensitive Ion Channels to Membrane Strain and Tension
; ...currents. Nonselective mechanosensitive cation channels with a conductance of 15 pS were observed...deformation involved stretch-activated cation channels and was sensitive to surface tensile...more in detail. Stretch-activated cation channels were originally discovered by Guharay...
Ca^sup 2+^-dependent Regulation of Phototransduction[dagger]
; ...pool induces closure of cGMP-gated cation channels and hyperpolarization of photoreceptor...cytoplasmic cGMP and closing cGMP-gated cation channels. The level of cGMP is then restored...determined, in part, by open cGMP-gated cation channels. The fraction of open channels depends...
On the Inside
; ...cold-induced, nonselective Ca^sup 2+^-permeable cation channels in Arabidopsis. This channel was not mechanosensitive...to be different from other previously described cation channels in Arabidopsis. As a first step toward finding...
Plants do it differently. A new basis for potassium/sodium selectivity in the pore of an ion channel
; ...CNGCs). Animal CNGCs are nonselective cation channels that do not discriminate between Na...voltage-independent) nonselective cation channels (Tyerman and Skerrett, 1999; Davenport...CNGCs). Animal CNGCs are nonselective cation channels that are activated (allosterically...
cAMP Modulation of the Cytoplasmic Domain in the HCN2 Channel Investigated by Molecular Simulations
; ...hyperpolarization-activated cyclic nucleotide-modulated (HCN) cation channels are opened by membrane hyperpolarization, while their activation...hyperpolarization-activated cyclic nucleotide-modulated (HCN) cation channels family includes, in vertebrates, four members (HCN1-4) involved...
Nonselective cation channels. Multiple functions and commonalities
; ...December issue, five papers address aspects of nonselective cation channels that includes their roles in salinity stress, biophysical...channels investigated have been referred to as nonselective cation channels (NSCCs) or voltage-independent channels (VICs). The latter...
Malate-permeable channels and cation channels activated by aluminum in the apical cells of wheat roots
; Malate-Permeable Channels and Cation Channels Activated by Aluminum in the Apical Cells of Wheat Roots1...capacity for Al" to activate malate permeable channels and cation channels for sustained malate release. When aluminum (Al) is solubilized...
Cold Transiently Activates Calcium-Permeable Channels in Arabidopsis Mesophyll Cells1[W]
; ...belong to the transient receptor potential superfamily of cation channels. These channels function as detectors of chemical and physical...transient cold-induced nonselective Ca^sup 2+^-permeable cation channels in plants. RESULTS Repetitive CIPCs in Arabidopsis Mesophyll...
Sodium uptake in Arabidopsis roots is regulated by cyclic nucleotides
; ...However, inward- and outward-- rectifying voltage dependent cation channels were generally found to be highly selective for K+ with...recently it has been suggested that voltage-- independent cation channels (VICs) identified in species such as wheat (Triticum aestivum...