Transformation of lepidopteran and coleopteran insect cell lines by Glyptapanteles indiensis polydnavirus DNA

From: In Vitro Cellular & Developmental Biology | Date: February 1, 1999| Author: | Copyright information

SUMMARY

iecently investigators showed that polydnavirus DIVA trom the parasitic wasp (7typtapante es iensis could transtorm gypsy moth L. dispar cell lines in vitro (McKelvey et al., 1996). Here we show GiPDV DNA is capable of transforming in vitro to varying degrees lepidopteran (IPLB-TN-R2, IPLB-SF-21, IAL-PID2, IPLB-HvTl) and coleopteran (IPLB-DU182E) insect cell lines derived from various somatic tissue types. An insect cell line derived from dipteran Aedes albopictus (C7/ 10) could not be transformed with G. indiensis polydnavirus. Key words: in vitro; parasitic wasp; Lymantria dispar.

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