Advanced analysis of biotin metabolites in body fluids allows a more accurate measurement of biotin bioavailability and metabolism in humans

The Journal of Nutrition | February 1, 1999| | Copyright

Advanced Analysis of Biotin Metabolites in Body Fluids Allows a More Accurate Measurement of Biotin Bioavailability and Metabolism in Humans w2

In previous studies, the bioavailability of biotin in humans was estimated from the recovery of biotin in urine; urinary biotin was measured by microbial growth assays or assays of avidin-binding activity. These assays underestimate concentrations of biotin metabolites, which originate from 3-oxidation, sulfur oxidation or a combination. We have developed an HPLC/avidin-binding assay that is specific for biotin and its metabolites. With the use of ...

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