Tryptophan scanning of D1S6 and D4S6 C-termini in voltage-gated sodium channels

From: Biophysical Journal | Date: August 1, 2003| Author: | Copyright information

ABSTRACT Recent reports suggest that four S6 C-termini may jointly close the voltage-gated cation channel at the cytoplasmic side, probably as an inverted teepee structure. In this study we substituted individually a total of 18 residues at D1S6 and D4S6 C-terminal ends of the rNav1.4 Na+ channel [alpha]-subunit with tryptophan (W) and examined their corresponding gating properties when expressed in Hek293t cells along with [nu]1 subunit. Several W-mutants displayed significant changes in activation, fast inactivation, and/or slow inactivation gating. In particular, five S6 W-mutants showed ...

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