(Alpha)-Bungarotoxin binding to acetylcholine receptor membranes studied by low angle x-ray diffraction

From: Biophysical Journal | Date: August 1, 2003| Author: | Copyright information

ABSTRACT The nicotinic acetylcholine receptor (nAChR) carries two binding sites for snake venom neurotoxins. [alpha]-Bungarotoxin from the Southeast Asian banded krait, Bungarus multicinctus, is a long neurotoxin which competitively blocks the nAChR at the acetylcholine binding sites in a relatively irreversible manner. Low angle x-ray diffraction was used to generate electron density profile structures at 14-[Angstrom] resolution for Torpedo caiifornica nAChR membranes in the absence and presence of [alpha]-bungarotoxin. Analysis of the lamellar diffraction data indicated a 452-[Angstrom] ...

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