Structural studies of MS2 bacteriophage virus particle disassembly by nuclear magnetic resonance relaxation measurements From: Biophysical Journal | Date: June 1, 2003| Author: | Copyright Biophysical Society Jun 2003. Provided by ProQuest LLC.Copyright information

ABSTRACT In this article we studied, by nuclear magnetic resonance relaxation measurements, the disassembly of a virus particle-the MS2 bacteriophage. MS2 is one of the single-stranded RNA bacteriophages that infect Escherichia coli. At pH 4.5, the phage turns to a metastable state, as is indicated by an increase in the observed nuclear magnetic resonance signal intensity upon decreasing the pH from 7.0 to 4.5. Steady-state fluorescence and circular dichroism spectra at pH 4.5 show that the difference in conformation and secondary structure is not pronounced if compared with the phage at pH ...

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

	Dependence of M13 major coat protein oligomerization and lateral segregation on bilayer composition ; ABSTRACT M13 major coat protein was derivatized with BODIPY (n...DOPC) are formed. INTRODUCTION M13 coat protein has been shown to exist in many...preferential association between M13 coat protein and/or lipids (Hemminga et al...
	Physical Regulation of the Self-Assembly of Tobacco Mosaic Virus Coat Protein ; ...in vitro aggregation behavior of the coat protein of tobacco mosaic virus (TMV). By comparing...different kinds of aggregated state of the coat protein. These are hydrophobic interactions...particles in aqueous solutions of the coat protein (CP) of tobacco mosaic virus (TMV) is...
	Lipid Bilayer Topology of the Transmembrane [alpha]-Helix of M13 Major Coat Protein and Bilayer Polarity Profile by Site-Directed Fluorescence Spectroscopy ; ...cysteine mutants of M13 major coat protein incorporated in lipid...transmembrane helix of the coat protein turns out to be 23 4...diffraction or solution nuclear magnetic resonance (NMR), encounter...membrane-bound state of the major coat protein of bacteriophage M13 ...
	FRET Study of Membrane Proteins: Determination of the Tilt and Orientation of the N-Terminal Domain of M13 Major Coat Protein ; ...of the N-terminal domain of the major coat protein from bacteriophage M13 reconstituted...2-dimyristoleoyl-sn-glycero-3-phosphocholine. INTRODUCTION M13 major coat protein is a small protein composed of 50 amino...intensive studies, the topology of the coat protein in lipid bilayers ...
	Resistance to Alfalfa Mosaic Virus in Transgenic Burley Tobaccos Expressing the AMV Coat Protein Gene. ; ...Stark and Beachy, 1989). Indeed, the AMV coat protein (CP) gene has been transformed into...studies imply that introducing a viral coat protein gene into tobacco may serve as an effective...RNA requires the addition of RNA 4 or coat protein (Bol et al., 1971). Thus, the coat ...
	Agronomic Performance of Transgenic Burley Tobaccos Expressing TVMV or AMV Coat Protein Genes with and without Virus Challenges.(tobacco vein mottling vires, alfalfa mosaic virus)(Statistical Data Included) ; ...tests that assessed the effectiveness of coat protein gene-mediated resistance (CPMR) in several...transgenic lines engineered for a viral coat protein gene may not only affect the direct...tobacco genotypes were transformed with a coat protein gene obtained from TVMV, and three of...
	Detecting the magnetic force of protons. (nuclear magnetic resonance used to image individual molecules) (Brief Article) ; ...magnetic forces can be used to detect nuclear magnetic resonance. Although this achievement...June 10 SCIENCE. The notion of using nuclear magnetic resonance for imaging individual molecules...The results ... demonstrate that [nuclear magnetic resonance] force detection can achieve...
	Nuclear magnetic resonance of polymers.(Understanding structure-property relationships in polymers)(Brief Article) ; Nuclear magnetic resonance of polymers. Structural studies of polymers by solution...polymers provides an overview of the methods and applications of nuclear magnetic resonance to relevant polymer problems with emphasis on how NMR...
	An early tobacco mosaic virus-induced oxidative burst in tobacco indicates extracellular perception of the virus coat protein ; ...was not a prerequisite for this elicitation and isolated coat protein (CP) subunits could also elicit the fast oxidative burst...single-stranded plus-sense RNA molecule packaged inside a rigid rod of coat protein (CP). The virus probably enters a plant passively through...
	Deuterium/hydrogen exchange factors measured by solution nuclear magnetic resonance spectroscopy as indicators of the structure and topology of membrane proteins ; ...of the 50-residue fd coat protein and the 23-residue magainin2...micelles by solution nuclear magnetic resonance spectroscopy...membrane-associated polypeptides. In fd coat protein, residues in the transmembrane...structure determination by nuclear magnetic resonance ...

For more facts and information, see all results