Culture of human main pancreatic duct epithelial cells From: In Vitro Cellular & Developmental Biology | Date: March 1, 1998| Author: | Copyright Society for In Vitro Biology Mar 1998. Provided by ProQuest LLC.Copyright information

SUMMARY

Attempts to grow human pancreatic duct epithelial cells in long-term culture have proven difficult. We have developed a system of growing these cells for several passages by adapting methods used to culture dog pancreatic duct cells. Epithelial cells were enzymatically dissociated from the main pancreatic duct and plated onto collagen-coated culture inserts suspended above a human fibroblast feeder layer. After primary culture, the cells were either passaged onto new inserts or plastic tissue culture plates in the absence of collagen. Cells grown on the latter plates were maintained ...

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