Determination of [beta]-glucan molecular weight using SEC with calcofluor detection in cereal extracts

Cereal Chemistry | July 1, 2003| | Copyright

ABSTRACT

A high-performance size-exclusion chromatography system (HPSEC) was set up with detection based on the specific binding of Calcofluor to [beta]-glucan for determination of amount and molecular weight of [beta]-glucan in different cereal extracts. To calibrate the HPSEC system, a purified [beta]-glucan was fractionated into narrow molecular weight ranges and the average molecular weight was determined before analysis on the HPSEC system. The detector response was similar for [beta]-glucans from oats and barley and appeared to be independent of molecular weight. Four differ-ent methods ...

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