Tensile force-dependent neurite elicitation via anti-[beta]1 integrin antibody-coated magnetic beads

From: Biophysical Journal | Date: July 1, 2003| Author: | Copyright information

ABSTRACT Previous work using glass microneedles to apply calibrated, localized force to neurons showed that tensile force is a sufficient signal for neurite initiation and elongation. However, previous studies did not examine the kinetics or probability of neurite initiation as a function of forc e or the rate of force application. Here we report the use of a new technique-magnetic bead force application-to systematically investigate the role of force in these phenomena with better ease of use and control over force than glass microneedles. Force-induced neurite initiation from embryonic chick ...

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