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The N-terminal amino acid sequences of the firefly luciferase are important for the stability of the enzyme
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Deukyong Sung and Hunseung Kang*
ABSTRACT
The structural and catalytic role of the N-terminal amino acid sequence of Photinus pyralis luciferase was investigated by site-directed mutagenesis. The firefly luciferase activity of a series of deletion and site-directed mutants in the amino-terminal region was investigated in vitro and in vivo. The mutant luciferases were produced either by in vitro transcription and translation or by expressing the cDNA encoding firefly luciferase in the yeast Saccharomyces cerevisiae as a fusion protein to the galactose DNA binding domain protein. Deleting the ...
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Rapid degradation of auxin/indoleacetic acid proteins requires conserved amino acids of domain II and is proteasome dependent
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