Imaging of adenoviral-directed herpes simplex virus type 1 thymidine kinase reporter gene expression in mice with radiolabeled ganciclovir

From: The Journal of Nuclear Medicine | Date: November 1, 1998| Author: Gambhir, Sanjiv S; Barrio, Jorge R; Wu, Lily; Iyer, Meera; Et al | Copyright information

We are developing procedures to repeatedly and noninvasively image the expression of transplanted reporter genes in living animals and in patients, using PET. We have investigated the use of the Herpes Simplex Virus type 1 thymidine kinase gene (HSV1-tk) as a reporter gene and [8-^sup 14^C]-ganciclovir as a reporter probe. HSV1-tk, when expressed, leads to phosphorylation of [8-^sup 14^C]-ganciclovir. As a result, specific accumulation of phosphorylated [8-^sup 14^C]-ganciclovir should occur ...

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