Probing the electrostatic shielding of DNA with capillary electrophoresis

From: Biophysical Journal | Date: March 1, 2003| Author: | Copyright information

ABSTRACT The free solution mobility of a 20-bp double-stranded DNA oligomer has been measured in diethylmalonate (DM) and Tris-acetate buffers, with and without added NaCl or TrisCl. DM buffers have the advantage that the buffering ion is anionic, so the cation composition in the solution can be varied at will. The results indicate that the free solution mobility of DNA decreases linearly with the logarithm of ionic strength when the ionic strength is increased by increasing the buffer concentration. The mobility also decreases linearly with the logarithm of ionic strength when NaCl is added ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

A simple method for determination of volatile acid concentration with corrections for ionic strength
; ...previously proposed to account for ionic strength and combines several volatile acid...the original method to incorporate ionic strength effects to increase the procedure's...without error and with the Davies ionic strength correction). Six simulated samples...
Fabrication of high-value particle assemblies.(Technical)
; ...particles, (b) we introduce a high ionic strength to start diffusion-limited aggregation caused by van der Waals forces. (c) The ionic strength is quickly diluted after roughly the...chemically fuse them. Why does raising the ionic strength allow doublets to form? A common way...
Electrostatic Steering at Acetylcholine Binding Sites
; ...AChE active site, in physiological ionic strength conditions. The potential at the...determining the local potential in increasing ionic strength, Debye-Hckel theory predicted that...of two- to sixfold (3), depending on ionic strength. These changes were consistent with...
Marinades 'Meat' Challenges.(Brief Article)
; ...lower salt levels that don't have the ionic strength necessary to provide the water-holding...We are investigating ways to improve ionic strength without impacting sensory attributes. Ionic strength is a key factor in marinade retention...
[CI.sup.-] and [Glutamate.sup.-] competition for a volume-regulated anion channel.
; ...in the presence and absence of an ionic strength gradient. Human embryonic kidney...HC1 or N-methyl D-glutamine. High ionic strength external solutions contained 150 CF or Glut; low ionic strength solutions contained 150 [Cl.sup...
Ionic interactions play a role in the regulatory mechanism of scallop heavy meromyosin
; ...data show that K^sub eq^ is markedly ionic strength dependent, with high salt (> or...and the effects of ADP, calcium and ionic strength were in qualitative agreement. Thus...with nucleotides was influenced by the ionic strength. The dissociation rate constants for...
Darcy Permeability of Agarose-Glycosaminoglycan Gels Analyzed Using Fiber-Mixture and Donnan Models
; ...were GAG concentration and solution ionic strength. The addition of even small amounts...open-circuit permeability when the ionic strength was decreased from 1 M to 0.01 M. A...predicted the Darcy permeability when the ionic strength was high enough to suppress the effects...
Role of a Conserved Salt Bridge between the PAS Core and the N-Terminal Domain in the Activation of the Photoreceptor Photoactive Yellow Protein
; ABSTRACT The effect of ionic strength on the conformational equilibrium...direction of I'^sub 2^. At higher ionic strength both effects reverse. Experiments...low salt effect depends on the ionic strength and not on the cation or anion...
Detection of red cell antibodies: comparison of two low ionic strength diluents
; ABSTRACT Various low ionic strength diluents are used routinely for...agglutination. Two commercial low ionic strength diluents (DiaMed ID-CellStab...detection. Effects of two low ionic strength diluents after 15-min incubation...
Competing Hydrophobic and Screened-Coulomb Interactions in Hepatitis B Virus Capsid Assembly
; ...temperature interval, an increase in ionic strength up to five times that of standard physiological...temperature virtually independently of the ionic strength, and that, at fixed temperature, the...strength increases with the square root of ionic strength. Both predictions are in ...