Secretion trap tagging of secreted and membrane-spanning proteins using Arabidopsis gene traps1

From: Plant Physiology | Date: June 1, 2003| Author: | Copyright information

Secreted and membrane-spanning proteins play fundamental roles in plant development but pose challenges for genetic identification and characterization. We describe a "secretion trap" screen for gene trap insertions in genes encoding proteins routed through the secretory pathway. The gene trap transposon encodes a [beta]-glucuronidase reporter enzyme that is inhibited by N-linked glycosylation specific to the secretory pathway. Treatment of seedlings with tunicamycin inhibits glycosylation, resulting in increased activity of secreted [beta]-glucuronidase fusions that result from gene trap ...

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