Strategies for development of functionally equivalent promoters with minimum sequence homology for transgene expression in plants: Cis-elements in a novel DNA context versus domain swapping1

From: Plant Physiology | Date: June 1, 2003| Author: | Copyright information

The cauliflower mosaic virus 35S (35S) promoter has been extensively used for the constitutive expression of transgenes in dicotyledonous plants. The repetitive use of the same promoter is known to induce transgene inactivation due to promoter homology. As a way to circumvent this problem, we tested two different strategies for the development of synthetic promoters that are functionally equivalent but have a minimum sequence homology. Such promoters can be generated by (a) introducing known cis-elements in a novel or synthetic stretch of DNA or (b) "domain swapping," wherein domains of one ...

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