Rapid attainment of a doubled haploid line from transgenic maize (Zea mays L.) plants by means of anther culture From: In Vitro Cellular & Developmental Biology | Date: March 1, 2003| Author: Peter, S O; Schmid, J E; Stamp, P; Aulinger, I E | Copyright Society for In Vitro Biology Mar/Apr 2003. Provided by ProQuest LLC.Copyright information

SUMMARY

We present a strategy for establishing a transgenic doubled haploid maize line from heterozygous transgenic material by means of anther culture. Compared to conventional inbreeding, the in vitro androgenesis technique enables a faster generation of virtually fully homozygous lines. Since the androgenic response is highly genotype-dependent, we crossed transgenic, non-androgenic plants carrying a herbicide resistance marker gene (pat, encoding for phosphinothricin acetyl transfe...

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