Quantitative analysis of circulating mitochondrial DNA in plasma

From: Clinical Chemistry | Date: May 1, 2003| Author: | Copyright information

Clinical Chemistry 49:5 719-726 (2003)

Background: Recent studies have demonstrated the existence of circulating mitochondrial DNA in plasma and serum, but the concentrations and physical characteristics of circulating mitochondrial DNA are unknown. The aim of this study was to develop an assay to quantify mitochondrial DNA in the plasma of healthy individuals.

Methods: We adopted a real-time quantitative PCR approach and evaluated the specificity of the assay for detecting mitochondrial DNA with a cell line ([rho]^sup 0^) devoid of mitochondria. The concentrations and physical ...

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