Technical note: Use of RFLP to characterize Lactococcus lactis strains producing exopolysaccharides

From: Journal of Dairy Science | Date: April 1, 2003| Author: | Copyright information

ABSTRACT

Restriction fragment length polymorphism (RFLP) is used to differentiate microorganisms by analysis of their DNA restriction patterns. A modified RFLP procedure is proposed for the rapid characterization of Lactococcus lactis strains producing exopolysaccharides (EPS). The availability of such effective cataloging system is likely to benefit research aimed at identifying lactococcal strains that produce novel EPS.

(Key words: exopolysaccharide, Lactococcus lactis, restriction fragment length polymorphism)

Abbreviation key: EPS = exopolysaccharide, EPS^sup +^ = ability to produce ...

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