Heterogeneity of diffusion inside microbial biofilms determined by fluorescence correlation spectroscopy under two-photon excitation

From: Photochemistry and Photobiology | Date: June 1, 2002| Author: | Copyright information

Heterogeneity of Diffusion Inside Microbial Biofilms Determined by Fluorescence Correlation Spectroscopy Under Two-photon Excitation(para)

ABSTRACT

Fluorescence correlation spectroscopy (FCS) under two-- photon excitation was applied successfully to characterize the penetration and diffusion capabilities of fluorescent probes (latex beads and fluorescein isothiocyanate-dextran) of different size and electrical charge in two models of monomicrobial biofilms with low (Lactococcus lactis biofilm) or high (Stenotrophonas maltophilia biofilm) contents of extracellular polymeric substance (EPS). ...

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