Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, pyrocystis lunula From: Photochemistry and Photobiology | Date: March 1, 2002| Author: | Copyright American Society of Photobiology Mar 2002. Provided by ProQuest LLC.Copyright information

Cloning and Characterization of an Active Fragment of Luciferase from a Luminescent Marine Alga, Pyrocystis lunula(para)

ABSTRACT

Two marine dinoflagellates, Lingulodinium polyedrum and Pyrocystis lunula, emit light in a reaction involving the enzymatic oxidation of its tetrapyrrole luciferin by molecular oxygen. The characteristic properties of P. lunula luciferase have not been clarified, whereas L. polyedrum luciferase, which has three active domains, has been characterized. A cloned partial cDNA of the P. lunula luciferase encodes an active fragment corresponding to part of domain 2 and ...