Polyvalent cations as permeant probes of MIC and TRPM7 pores

From: Biophysical Journal | Date: April 1, 2003| Author: | Copyright information

ABSTRACT Recent studies in Jurkat T cells and in rat basophilic leukemia cells revealed an Mg^sup 2+^-inhibited cation (MIC) channel that has electrophysiological properties similar to TRPM7 Eyring rate model expressed exogenously in mammalian cells. Here we compare the characteristics of several polyvalent cations and Mg^sup 2+^ to block monovalent MIC current from the outside. Putrescine, spermidine, spermine, PhTX-343 (a derivative of the naturally occurring polyamine toxin philanthotoxin), and Mg^sup 2+^ each blocked in a dose- and voltage-dependent manner, indicating a blocking site ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

Low-Affinity Na^sup +^ Uptake in the Halophyte Suaeda maritima1[C][W][OA]
; ...that cAMP and Ca^sup 2+^ (blockers of nonselective cation channels) and...inhibitor of Na^sup +^ uptake) did...suggest that neither nonselective cation channels nor a...pathways for Na^sup +^ entry into...suggest that Na^sup +^ influx across...voltage-independent ...
Sodium fluxes through nonselective cation channels in the plasma membrane of protoplasts from Arabidopsis roots
; Sodium Fluxes through Nonselective Cation Channels in the Plasma...was to characterize Na^sup +^ currents through nonselective cation channels (NSCCs) in protoplasts...protoplasts in low external Ca^sup 2+^ by digesting tissue...that actively remove Na^sup +^ from the ...
Pharmacokinetics and Biodistribution of ^sup 111^In- and ^sup 177^Lu-Labeled J591 Antibody Specific for Prostate-Specific Membrane Antigen: Prediction of ^sup 90^Y-J591 Radiation Dosimetry Based on ^sup 111^In or ^sup 177^Lu?
; ^sup 111^In-Labeled antibodies and peptides have...used as chemical and biologic surrogates for ^sup 90^Y-labeled therapeutic agents. However, recent...significant differences in biodistribution between ^sup 111^In- and ^sup 90^Y-labeled agents. Yttrium...
^sup 99m^Tc-MAEC Complexes: New Renal Radiopharmaceuticals Combining Characteristics of ^sup 99m^Tc-MAG3 and ^sup 99m^Tc-EC
; ^sup 99m^Tc-Mercaptoacetyltriglycine (^sup 99m^Tc-MAG3) and ^sup 99m^Tc-L,L-ethylenedicysteine (^sup 99m^Tc-LL-EC) are useful renal radiopharmaceuticals; however, both agents have renal clearances less than that of ^sup 131^I-orthoiodohippurate...
Effects of Intracellular and Extracellular Concentrations of Ca^sup 2+^, K+, and Cl- on the Na+-Dependent Mg^sup 2+^ Efflux in Rat Ventricular Myocytes
; ABSTRACT Intracellular Mg^sup 2+^ concentration ([Mg^sup 2+^]^sub i^) was measured in rat ventricular myocytes with...indicator furaptra (25C). After the myocytes were loaded with Mg^sup 2+^, the initial rate of decrease in [Mg^sup 2+^]^sub i^ (initial...
Development of a Novel ^sup 99m^Tc-Chelate-Conjugated Bisphosphonate with High Affinity for Bone as a Bone Scintigraphic Agent
; In bone scintigraphy using ^sup 99m^Tc with methylenediphosphonate (^sup 99m^Tc-MDP) and hydroxymethylenediphosphonate (^sup 99m^Tc-HMDP), it takes 2-6 h after an injection before imaging can start. This interval could be shortened with a new radiopharmaceutical...
Processing of Generator-Produced ^sup 68^Ga for Medical Application
; The ^sup 68^Ge/^sup 68^Ga generator provides an excellent source of positron-emitting ^sup 68^Ga. However, newly available "ionic" ^sup 68^Ge/^sup 68^Ga radionuclide generators are not necessarily optimized for the synthesis of ^sup 68^Ga-labeled...
^sup 203^Pb-Labeled [alpha]-Melanocyte-Stimulating Hormone Peptide as an Imaging Probe for Melanoma Detection
; Peptide-targeted α-therapy with 7.4 MBq of ^sup 212^Pb-[1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid]-ReO-[Cys^sup 3,4,10^, D-Phe^sup 7^,Arg^sup 11^]α-MSH^sub 3-13^ (^sup 212^Pb-DOTA-Re(Arg^sup 11^)CCMSH...
^sup 99m^Tc-N-DBODC5, a New Myocardial Perfusion Imaging Agent with Rapid Liver Clearance: Comparison with ^sup 99m^Tc-Sestamibi and ^sup 99m^Tc-Tetrofosmin in Rats
; ^sup 99m^Tc-[bis (dimethoxypropylphosphinoethyl)-ethoxyethylamine (PNP5...myocardial uptake and clearance kinetics and organ biodistribution of ^sup 99m^Tc-N-DBODC5 with ^sup 99m^Tc-sestamibi and ^sup 99m^Tc-tetrofosmin. Methods: Seventy-five...
NH^sub 4^^sup +^ Currents across the Peribacteroid Membrane of Soybean. Macroscopic and Microscopic Properties, Inhibition by Mg^sup 2+^, and Temperature Dependence Indicate a SubpicoSiemens Channel Finely Regulated by Divalent Cations1
; ...cycles of NH^sub 4^^sup +^/ammonia transport. An unusual nonselective cation channel with subpicoSiemens...permeable to NH^sub 4^^sup +^ had previously...regulation of the PBM-nonselective cation channel that could...constant NH^sub 4^^sup +^ gradient across...However, NH^sub ...