Creation of a thermostable firefly luciferase with pH-insensitive luminescent color

From: Photochemistry and Photobiology | Date: March 1, 2003| Author: | Copyright information

Creation of a Thermostable Firefly Luciferase with pH-insensitive Luminescent Colors

ABSTRACT

The thermal instability and pH-sensitive spectral property of firefly luciferase have hampered its use as a sensitive multicolor luminescent label or bioluminescent resonance energy transfer donor. With the intention of improving the thermostability of a previously found firefly Hotaria parvula luciferase mutant with minor pH-sensitive spectral change (V368A), further mutation (E356R) was introduced by taking a reportedly stabilized mutant of Photinus pyralis luciferase into account. The double ...

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