Rapid detection of (alpha)(+) thalassaemia deletion & (alpha)-globin gene triplication by Gap-PCR in Indian subjects

From: Indian Journal of Medical Research | Date: October 1, 2002| Author: | Copyright information

Background & objectives: Southern blot hybridization is the commonly used method to delineate (alpha) globin gene defects. This technique is time consuming, requires a large amount of genomic DNA and radioactive probes for detecting the mutations, which limits its use in diagnosis. The present paper emphasizes the efficacy of a well-established Gap-PCR technique in the Indian set up to detect defects of the (alpha) globin gene in clinics and laboratories engaged in the diagnosis of thalassaemias.

Methods: A total of 190 normal subjects (voluntary blood donors), 183 individuals with ...

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