Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: Identification of genes expressed differentially in epidermal cells or vascular tissues of maize From: Plant Cell | Date: March 1, 2003| Author: | Copyright American Society of Plant Physiologists Mar 2003. Provided by ProQuest LLC.Copyright information

GENOMICS ARTICLE

Laser-Capture Microdissection, a Tool for the Global Analysis of Gene Expression in Specific Plant Cell Types: Identification of Genes Expressed Differentially in Epidermal Cells or Vascular Tissues of Maize(W)

Laser-capture microdissection (LCM) allows for the one-step procurement of large homogeneous populations of cells from tissue sections. In mammals, LCM has been used to conduct cDNA microarray and proteomics studies on specific cell types. However, LCM has not been applied to plant cells, most likely because plant cell walls make it difficult to separate target cells ...

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

	Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei1[W][OA] ; ...different cell types and furthermore...patterns of gene expression that underlie...different cell types in purified form prior to gene expression measurements...targeted cell types. The application...regulation of gene expression is ...
	A Gene Regulatory Network Model for Cell-Fate Determination during Arabidopsis thaliana Flower Development That Is Robust and Recovers Experimental Gene Expression Profiles(W) ; ...states that match gene expression profiles observed...required to restrain cell types to those found...show that the cell types recovered depend...combination of gene expression states that specifies...lead to multiple cell types, each characterized...set ...
	Early gene expression associated with the commitment and differentiation of a plant tracheary element is revealed by cDNA-amplified fragment length polymorphism analysis ; Early Gene Expression Associated with the Commitment and Differentiation...synchrony. To reveal the changing patterns of gene expression during the 48 h of transdifferentiation from mesophyll to TE cell fate, we used a cDNA-amplified fragment length...embryonic cells differentiate into the 40 ...
	Toward a Systems Biology of Mouse Inner Ear Organogenesis: Gene Expression Pathways, Patterns and Network Analysis ; ...most comprehensive study to date on gene expression during mouse inner ear (IE) organogenesis...specification. Additional, clear-cut, gene expression signatures marked specific structures...hearing loss. There are numerous other cell types in the IE that are also required ...
	MAIZEWALL. Database and Developmental Gene Expression Profiling of Cell Wall Biosynthesis and Assembly in Maize 1[W] ; ...for each entry and gene expression data that are accessible...cell wall-related gene expression in different organs...cell wall-related gene expression throughout the maize...involving specific gene family members and...diversity of lignified cell ...
	Noninvasive Monitoring of Target Gene Expression by Imaging Reporter Gene Expression in Living Animals Using Improved Bicistronic Vectors ; ...noninvasive imaging of therapeutic gene expression based on levels of reporter gene expression is a powerful tool to devise improved...IRES in mediating downstream reporter gene expression in cell culture and in vivo. Methods...
	Gene expression: better tools: more complex results.(Cover story) ; Gene expression has become a mainstream technique...drug development. Broadly speaking, gene expression comes in two flavors: analytical...diseased/normal). With functional gene expression, the aim is to obtain some quantity...
	Tackling High Variability in Gene Expression Studies. ; ...Guilherme J. M. Rosa High variability in gene expression studies often prevents differences...are enhancing the ability to measure gene expression. Each method poses unique limitations...suggestions to tackle high variability in gene expression profiling. Tools for measuring ...
	Comparative immunocytochemistry of isolated rat & monkey pancreatic islet cell types ; ...comparative distribution of the islet cell types in the Indian bonnet monkeys and rats...compare the arrangement of the three islet cell types in the native and in isolated rat and...revealed the presence of three different cell types: insulin secreting B cells, glucagon...
	Toward a Neutral Evolutionary Model of Gene Expression ; ...that describes neutral changes of gene expression over evolutionary time as a compound...deviation from clock-like behavior of gene expression distances. Model-based analyses of gene expression profiles in primate liver and brain...

For more facts and information, see all results